Basic principles of peptide synthesis

Solid phase synthesis of peptides is a major breakthrough in peptide synthesis chemistry. Its most important feature is that it is not necessary to purify the intermediates, and the synthesis process can be carried out continuously. At present, the synthesis of fully automated peptides is almost solid phase synthesis. The basic process is as follows:

Based on the chemical synthesis of Fmoc, the first to target C- terminal amino acid polypeptide synthesis of carboxyl groups covalently linked with an insoluble polymer, then the amino acid as a starting point for peptide synthesis and amino acids from its carboxyl (activated) role in the formation of peptide bond, repeating this a process to obtain a target polypeptide. According to the amino acid composition of the polypeptide, the way of the peptide was different and the purification method was different.

Solid phase synthesis of peptides is a major breakthrough in peptide synthesis chemistry. Its most important feature is that it is not necessary to purify the intermediates, and the synthesis process can be carried out continuously. At present, the synthesis of fully automated peptides is almost solid phase synthesis. The basic process is as follows: Fmoc chemical synthesis based on the first to target C- terminal amino acid polypeptide synthesis of carboxyl groups covalently linked with an insoluble polymer, then the amino acid as a starting point for peptide synthesis and amino acids from its carboxyl (activated) role in the formation of peptide bonds and repeat this process, can obtain the target polypeptide. According to the amino acid composition of the polypeptide, the way of the peptide was different and the purification method was different.